| 徐红梅,夏仕文,何从林.固定化D-氨基酸氧化酶催化DL-氨基酸去消旋化制备非天然L-氨基酸[J].分子催化,2012,(2):192-196 |
| 固定化D-氨基酸氧化酶催化DL-氨基酸去消旋化制备非天然L-氨基酸 |
| Preparation of Non-natural L –amino Acids via Deracemization of DL-Amino Acids Catalyzed by Immobilized D-amino Acid Oxidase |
| 投稿时间：2012-02-14 修订日期：2012-03-14 |
| DOI： |
| 中文关键词: 固定化D-氨基酸氧化酶，过氧化氢酶，氧化脱氨，DL-氨基酸，非天然L-氨基酸 |
| 英文关键词:Immobilized D-amino acid oxidase Catalase Oxidative deamination DL-amino acids Non-natural amino acids |
| 基金项目:重庆市自然科学基金项目 |
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| 中文摘要: |
| 在过氧化氢酶和氧气存在下，固定化D-氨基酸氧化酶（D-AAO）对映选择性催化DL-氨基酸中的D-对映体氧化脱氨为相应酮酸，L-对映体保留。研究了D-AAO的底物特异性并对反应条件进行了优化。结果表明：D-AAO具有较宽的底物谱，能够催化疏水性α-氨基酸的D-对映体氧化脱氨。在最优反应条件下，D-AAO催化DL-2-氨基丁酸、DL-2-氨基戊酸去消旋化，L-2-氨基丁酸、L-2-氨基戊酸的收率分别为48%和47%，ee分别为99.5%和99.8%。进一步地，利用Pd-C/HCOONH4催化氧化脱氨过程中产生的亚氨基酸原位还原，有效提高了L-2-氨基丁酸、L-2-氨基戊酸的收率并保持高的光学纯度。 |
| 英文摘要: |
| The D-enantiomer of DL-amino acid is oxidative deaminated enantioselectively into the corresponding oxo acid by immobilized D-amino acid oxidase (D-AAO) in the presence of catalase and oxygen, and the L-enantiomer is retained. The substrate specificity of D-AAO was studied and the reaction conditions were optimized. The results showed that D-AAO has a broader substrate spectrum and is capable of catalyzing the oxidative deaminzation of hydrophobic D-α-amino acids. Under the optimal conditions, L-2-aminobutyric acid and L-2-aminovaleric acid were obtained in 48% and 47% yield , 99.5% and 99.8% ee respectively. Furthemore, the yields of L-2-aminobutyric acid and L-2-aminovaleric acid were improved and their optically purity were retained through in-situ reduction of imine acids produced during the oxidative deaminization by Pd-C/HCOONH4. |
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